Figure 2
From: In vitro and in vivo multidrug resistance reversal activity by a Betti-base derivative of tylosin
(A) Doxorubicin accumulation (in μg ml–1, assuming an average volume of 3 μl per 106 cells) in non-resistant (parental cells) and human MDR1 gene-transfected (transfected cells) L5178 mouse lymphoma cells. Cells were exposed to 40 μ M doxorubicin for 1 h (with or without 10 μ M TBN), after which they were extracted and the amount of doxorubicin quantified by LC. The values are mean ± s.d. of three independent experiments. (B) Effect of 40 μ M doxorubicin for 1 h (with or without 10 μ M TBN) on the proliferation of the different cells. After treatment, cells were washed and cultured for 48 h at 37°C using 96-well plates. The values are mean ± s.d. of three independent experiments (each conducted in triplicate). Significant differences between means, as specified by capped lines, are indicated by ***P<0.0001. ns, not significant.
