Table 1 FARs of the compounds calculated on the basis of the measured fluorescence values (see Materials and methods)

From: In vitro and in vivo multidrug resistance reversal activity by a Betti-base derivative of tylosin

Samples

Concentration ( μ m)

FAR ± s.d.

Verapamil

22

10±3.2

Betti-base

3.5

10±5.1

 

35

8±1.7

Tylosin

3.5

1±0.0

 

35

5±7.5

TBN

3.5

105±8.5

 

35

94±25.5

  1. Abbreviations: FAR=fluorescence activity ratio; TBN=N-tylosil-1-α-amino-(3-bromophenyl)-methyl-2-naphthol.
  2. Non-resistant (parental cells) and human MDR1 gene-transfected L5178 mouse lymphoma cells were treated with rhodamine 123 and the individual compounds for 20 min at 37°C. The fluorescence of the cell population was then measured with a flow cytometer.
  3. Mean and s.d. values were calculated from at least three independent experiments.
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