Figure 1
From: The tumour-suppressive miR-29a/b1 cluster is regulated by CEBPA and blocked in human AML
CEBPA mediates miR-29b expression in leukaemic cells. (A) Mature miR-29b expression was induced two-fold after restoring CEBPA function in leukemic Kasumi-1 cells. The CEBPA target miR-223 was used as positive control for restored CEBPA function. Results are given as fold expression compared with untreated cells (day 0, *P<0.05) and represent three independant experiments. (B) Conditional expression of AML1-ETO abolished CEBPA protein expression in U937 leukemic cells (B, left) and suppressed miR-29b expression (*P<0.05; B, right). miR-29b expression is given as fold changes compared with the control cells U937-T (ΔΔCt-method) and represent three independant experiments. (C) Protein levels of the putative miR-29b activator CEBPA and the miR-29b target DNMT3B inversely correlated in leukemic cell lines (C, left). miR-29b expression correlated to CEBPA, and inversely correlated to DNMT3B protein levels (C, right). miR-29b expression is given as ΔCt-values (Ct(miR-29b)−Ct(normalisation)) representing three independant experiments.
