Table 1 Antibodies characteristics
Antibody | Reference | Dilution | Ag retrieval (min) | Primary Ab (min) |
|---|---|---|---|---|
Anti-c-kit | A4502; Dako, Glostrup, Denmark | 1 : 100 | 30 | 24 |
Anti-PDGFR α | AF 307 NA; R&D, Minneapolis, MN, USA | 1 : 50 | 30 | 32 |
Anti-PDGFR β | sc-339; Santa Cruz, Santa Cruz, CA, USA | 1 : 300 | 30 | 32 |
Anti-M-CSFR | 49C10; Cell Signaling, Danvers, MA, USA | 1 : 50 | 30 | 32 |
Anti-E cadherin | 18-0223; Zymed, Carlsbad, CA, USA | 1 : 70 | 30 | 24 |
Anti-β catenin | M3539; Dako | 1 : 200 | 30 | 32 |
Anti-p42 map Kinase ERK | 9108; Cell Signaling | 1 : 100 | 30 | 32 |
Anti-phospho-AKT Ser 473 | 3787; Cell Signaling | 1 : 50 | 30 | 32 |
Anti-phospho-MEK 1/2 | 2338; Cell Signaling | 1 : 50 | 30 | 32 |
Anti-cyclin D1 | RM-9104-R7; Neomarkers, Fremont, CA, USA | 1 | 30 | 24 |
