Table 3 Sequences of primers used in real-time RT-PCR analysis
From: Wnt signalling in adenomas of familial adenomatous polyposis patients
| Â | Assay type | Primers |
|---|---|---|
Human PCR assays | ||
 sFRP1 (NM_003012) | TaqMan | F, 5′-CCAATCCCACCGAAGCCT-3′ |
|  |  | R, 5′-ATGATGGCCTCAGATTTCAACTC-3′ |
|  |  | P, 5′-CAAGCCCCAAGGCACAACGGTG-3′ |
 NKD1 (NM_033119) | TaqMan | F, 5′-TCGCCGGGATAGAAAACTACA-3′ |
|  |  | R, 5′-CAGTTCTGACTTCTGGGCCAC-3′ |
|  |  | P, 5′-CCAATTTGGGCCTGGCTCCCC-3′ |
 FZD3 (NM_017412) | TaqMan | F, 5′-CATGGAGATGTTTGGTGTTCCTT-3′ |
|  |  | R, 5′-AAGTCGAGGATATGGCTCATCAC-3′ |
|  |  | P, 5′-TCTGGGAACCTACTGCATTCCATATCTTCAGG-3′ |
 c-myc (NM_002467) | TaqMan | F, 5′-AGCTGCTTAGACGCTGGATTTT-3′ |
|  |  | R, 5′-TTCCTGTTGGTGAAGCTAACGTT-3′ |
|  |  | P, 5′-CAGCCTCCCGCGACGATGC-3′ |
 KRT8 (NM_002273) | TaqMan | F, 5′-GATCGCCACCTACAGGAAGCT-3′ |
|  |  | R, 5′-ACTCATGTTCTGCATCCCAGACT-3′ |
|  |  | P, 5′-CCGGCTCTCCTCGCCCTCCA-3′ |
