Figure 1 | British Journal of Cancer

Figure 1

From: Targeting pancreatic and ovarian carcinomas using the auristatin-based anti-CD70 antibody–drug conjugate SGN-75

Figure 1

Characterisation of anti-CD70 1C1 and 5D12. (A) Western blots containing FLAG-tagged CD70 ECD (lanes 1 and 5) and membrane extracts from HEK 293 cells (lanes 2 and 6), HEK 293:CD70 transfectants (lanes 3 and 7) and 786-O cells (lanes 4 and 8) were reacted with mAb 1C1 (left panel) or mAb 5D12 (right panel). The mAb 1C1 and 5D12 specifically bound recombinant CD70 (lane 1 and 4) and three distinct bands (*) from CD70+ membrane extracts (lanes 3, 4, 7, and 8). Extracts from parental HEK 293 cells were negative as expected (lane 2 and 6). The β-actin control shows comparable quantities of protein were loaded for each membrane extract (lower panels). (B) IHC analysis on FFPE normal human tissues showing limited expression of CD70 in lymphoid tissues. Red stain indicates presence of CD70+ cells. 1C1 mAb was used for these set of images. × 400 magnification, scale bar=50 μm.

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