Figure 3

Immunohistochemistry analysis using anti-CD70 mAb (1C1) in tumour microarrays. CD70 expression in pancreatic (A) and ovarian (B) tumours is shown. Of the 140 and 241 individual cases of pancreatic and ovarian carcinoma, respectively, 35 cases (25%) and 37 cases (15%) were found to be CD70+. In this analysis, the area of transformed cells within each biopsy stained positively for CD70 expression is categorised into <25%, 25–50%, 50–75%, and >75%. The intensity of CD70 expression is expressed as: 1=weak, 2=mild, 3=moderate, 4=strong ( × 400 magnification). To illustrate the heterogeneity in CD70 expression, the percent area positive is plotted against staining intensity. In these plots, the number in each in the graphs denotes the number of CD70+ cases. Representative images for low, heterogeneous (25–50% positive, 2+) and high, homogeneous (>75% positive, 4+) CD70 for pancreatic and ovarian carcinoma are shown.