Figure 4
In vitro and In vivo Anti-tumour Activity of SGN-75: (A and B) In vitro cytotoxicity assay showing potency of SGN-75 (triangles) on SK-OV-3 ovarian and CD70-transfected MiaPaCa-2 pancreatic carcinoma cell lines. The controls, unconjugated h1F6 control (
) and non-binding ADC (A, squares) have no effect. IC50 for SGN-75 and non-binding ADC are noted. Similarly, no cytotoxicity is observed in the untransfected MiaPaCa-2 control (B, squares). (C and D) In vivo anti-tumour activity of SGN-75 in0-transfected MiaPaCa-2 pancreatic carcinoma tumours in nude mice. Mice treated with SGN-75 (squares) showed a significantly reduced median tumour volume (C) and a higher percent survival (D) when compared with untreated (circles) and non-binding ADC (triangles).
