Figure 1

Protein tyrosine kinase 6–HER2 complex formation in T47D cells as visualised by the PLA technique. Control T47D cells (A, C) and T47D cells after siRNA-mediated down-regulation of PTK6 (B) or HER2 (D). Western blot analysis of proteins extracted from T47D cells after transfection with siRNA targeting PTK6 (E) and HER2 (F), respectively; control cells were treated with transfection reagents only or with siRNA targeting GAPDH; (tubulin=loading control).