Figure 9

InvColi-pS strains infect colon tissue samples and induce high COX-2 silencing associated with anti-inflammatory and anti-angiogenic effects. (A) COX-2 protein, COX-2 mRNA and siCOX-2 levels were analysed in colon tissue samples by western blot and real-time PCR, 48 h after InvColi strain infection. COX-2 protein, COX-2 mRNA and siCOX-2 expression was normalised against β-actin protein, GUSB (β-glucuronidase) mRNA and U6 RNA levels, respectively. Relative expression of COX-2 protein and COX-2 mRNA refers to not infected samples (negative control, NC); relative expression of siCOX-2 refers to InvColi-pS^TBE infected samples. Data represent mean±s.e.m. of three independent experiments. ^*P<0.01. (B) Levels of PGE₂, (C) cytokines, chemokines and growth factors were quantified in InvColi-pS⁻- and InvColi-pS^TBE-infected colon tissues conditioned media, 48 h after infection, by a Luminex-based multiplexed bead immunoassay. Concentration values are expressed as log(pg ml⁻¹) and represent mean±CV of three independent measurements, normalised considering the total protein amount extracted from each tissue sample. ^*P<0.01.