Figure 1
From: EVI1 controls proliferation in acute myeloid leukaemia through modulation of miR-1-2
EVI1 and miRNAs expression in AML. (A) Relative qRT–PCR quantification of EVI1 transcript expression in AML cell lines. The GADPH levels were used as a normaliser for the calculation of the 2−ΔΔCt coefficient. (B) Immunoblot analysis of EVI1 protein levels in nuclear (NE) and cytosolic fractions (CE). Lam A/C and tubulin levels serve as control for equal protein loading. (C) Identification of miRNAs differentially expressed (P<0.01) between EVI1+ and EVI1– cell lines by class comparison and significant analysis of microarrays (SAM) methods. (D) Statistical analysis of miR-1-2 and miR-133a-1 expression levels in EVI1+ and EVI1− cell lines. (E) Spearman's rank correlation coefficient between ΔCt EVI1 and ΔCt miR-1-2 or miR-133a-1 in patient samples.
