Figure 1
Epigenetic patterns in carcinoma in situ (CIS) testis visualised by immunohistochemistry. (A) A typical tubule with CIS cells marked by one of the classical markers, OCT4 (POU5F1) (de Jong et al, 2005; Rajpert-De Meyts et al, 2004). Note that CIS cells are bigger than unstained spermatogonia (visible in the tubule on the right) and have large irregular nuclei with coarse chromatin clumps. (B) Double staining for 5-methyl-cytosine (reddish brown) and the classical CIS marker placental-like alkaline phosphate (PLAP, dark blue). (C) H3K9me2. (D) Double staining for H3K27me3 (deep blue) and PLAP (reddish brown). (E) H3K4me1, (F) H3K4me2/3, (G) H3K9ac and (H) H2A.Z. Arrows denote CIS cells and arrowheads Sertoli cells. Bar represent 50 microns.
