Table 1 Characterisation of clinical material (A) and primary antibodies used for immunohistochemistry (IHC) (B)
(A) | |||
|---|---|---|---|
Diagnosis, FIGO | Histopathology | Abbrevation | Number of cases (%) |
Normal cervix | Normal cervix | SCE | 8 (11.94) |
CIN 2 | Moderate cervical intraepithelial dysplasia | CIN 2 | 4 (5.97) |
CIN 3 | Severe cervical intraepithelial dysplasia | CIN 3 | 9 (13.43) |
| Â | Squamous cervical carcinoma in situ | Â | 12 (17.91) |
SCC, stage IA1 | Microinvasion <3 mm in depth and <7 mm horizontally | MicSCC | 7 (10.45) |
SCC, stage IA2 | Invasion >5 mm in depth or >7 mm horizontally | InvSCC | 2 (2.99) |
SCC, stage IB1 | Visible lesion ≤4 cm in greatest dimension |  | 17 (25.37) |
SCC, stage IB2 | Visible lesion >4 cm |  | 2 (2.99) |
SCC, stage IIA | Without parametrial invasion, but involvimg upper 2/3 of vagina | Â | 1 (1.49) |
SCC, stage IIB | With parametrial invasion | Â | 1 (1.49) |
SCC, stage III | Extends to pelvic wall or lower third of vagina | Â | 3 (4.48) |
Total | Â | Â | 66 |
(B) | |||||||
|---|---|---|---|---|---|---|---|
Protein | Antibody | Origin | Clone | Epitope detection | Dilution factor | Tissues for positive controls | Manufacturer |
ANXA6 | Sc-1931 | Goat polyclonal | N-19 | N-terminus | 400 | Liver hepatocytes | SantaCruz (SantaCruz, CA, USA) |
HSP27 | Sc-13132 | Mouse monoclonal | F-4 | Amino acids 32–108 | 400 | Tonsilla | SantaCruz (SantaCruz, CA, USA) |
PRDX2 | WH007001 | Mouse monoclonal | 4E 10-2D2 | Amino acids 1–199 | 100 | Tonsilla | Sigma-Aldrich |
NCF2 | HPA006040 | Rabbit polyclonal | PrEST | Full-length | 150 | Glandular epithelium of colon | Sigma-Aldrich |
TPM4 | WH000717 | Mouse monoclonal | 4E4-1D2 | Full-length | 200 | Placenta, decidual tissue | Sigma-Aldrich |
