Figure 2

Time–course response for leptin-mediated effects on the expression of IL-1 system in 4T1 cells. Leptin effects on protein levels of IL-1α (A), IL-1β (B), IL-1Ra (C) as determined by ELISA. Levels of cytokines were normalised to basal condition (assigned as 1) and expressed in arbitrary units. IL-1R tI (D) as determined by western blot (WB). (E) WB results for IL-1R tI were normalised to β-actin as a control and densitometric analysis of bands was carried out with the imageJ software. 4T1 cells were cultured in a medium containing 1.2 nM leptin for 0–48 h. (a) P<0.05 when comparing levels of protein to control (basal). Data (mean±s.e.) are representative of the results derived from a minimum of three independent experiments.