Figure 6
From: Leptin pro-angiogenic signature in breast cancer is linked to IL-1 signalling
Leptin upregulated OB-R in 4T1 cells. Representative histograms from flow cytometric determination of leptin effects on the expression of OB-R in 4T1 cells. (A) Positive control (β-actin antibody) and negative control (isotype-matched unspecific IgG), (B) basal and leptin-induced expression of OB-R all isoforms (anti-OB-R NH2-terminus antibody), (C) basal and leptin-induced expression of OB-Rb (long isoform; anti-OB-Rb COOH-terminus antibody). 4T1 cells were cultured in medium containing 1.2 nM leptin for 24 h, incubated with specific antibodies and analyzed by flow cytometry. Data derived from a minimum of three independent experiments were analysed using BD FACSDiva and FlowJo software.
