Figure 6

Effect of HIF-1α suppression induced by lentivirus-delivered shRNA on the cell viability of gastric cancer cell lines SNU-668 (top), SNU-484 (middle), and SNU-216 (bottom). Cells were infected with a lentivirus containing a construct, which encodes either HIF-1α shRNA (denoted as shHIF-1α) or scrambled shRNA (denoted as LVcontrol). Expression levels of HIF-1α and β-actin proteins were determined by western blotting after cells were exposed to normoxia (N) or hypoxia (H) for 8 h (A). Cell viability was measured at the indicated time of hypoxia exposure and was represented as the percentage of cell number expressing HIF-1α shRNA vs vector control cells (B). Values represent the means±s.d. (n=4). *P<0.05.