Figure 1 | British Journal of Cancer

Figure 1

From: Diabetogenic glucose and insulin concentrations modulate transcriptom and protein levels involved in tumour cell migration, adhesion and proliferation

Figure 1

(A) Cell surface expression levels of α2 and β1 integrin, E-, N- and VE-cadherins on SW480 colon carcinoma cells. The antibody-bound FITC-fluorescence of specific antibodies at 11 mM glucose or 11 mM glucose plus insulin (filled grey) was directly compared with 5.5 mM control cells (grey line) as well as to the isotype control (black line). Significant increases (more than 15%) have been found for α2, β1integrins and for VE-cadherin. For N-cadherin only a marginal induction was detectable, whereas E-cadherin showed no significant changes. The geometric means (Gm) indicated the fluorescence intensity of the FITC-conjugated antibodies. (B) Immunoblot analysis of E-cadherin expression of breast cancer (MDA MB468) and colon carcinoma (SW480) cell lines. Antibodies against the cell–cell contact molecule E-cadherin were used, and specifically bound antibodies were detected with the use of a secondary HRP-linked mouse anti-human antibody. The expression levels of all blots were evaluated using the Wasabi software version 1.4 (Hamamatsu Photonics, Herrsching am Ammersee, Germany).

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