Figure 2

Analysis of the distance migrated of MDA MB468 breast cancer cells. Bars in (A) display the average distance (±s.e.m.) of 30 randomly selected cells of a 12 h observation period. Cells were cultured at either 5.5 mM or 11 mM glucose and treated with 100 ng ml⁻¹ insulin, 250 nM wortmannin or a combination of both substances. (B) Depicts the distance migrated and the average migratory activity of MDA MB468 cells cultured at either 5.5 mM or 11 mM glucose with and without additional insulin (100 ng ml⁻¹) and treated with the PI3K inhibitor wortmannin, the PKCα inhibitor Go6976, or the MLCK inhibitor ML-7. The results show mean values of three independent experiments. Statistical comparison between specified values was made by two-tailed unpaired t-test. ^*P<0.05; ^**P<0.01; ^***P<0.001; NS, not significant.