Table 2 Micro array analysis revealed alterations of gene activity by comparing physiological and diabetogenic conditions

From: Diabetogenic glucose and insulin concentrations modulate transcriptom and protein levels involved in tumour cell migration, adhesion and proliferation

 

5.5 m M vs 11 m M glucose

5.5 m M vs 11 m M glucose+insulin

Proliferation/cell cycle

 Cyclin A1

14.09

11.13

 Cyclin E2

4.90

2.39

 E2F transcription factor 3

4.16

4.80

Signal transduction

 Integrin-linked kinase

3.92

4.85

 Phosphoinositide-3-kinase

2.76

4.05

 Protein kinase Cα

4.68

9.71

 Protein kinase Cδ

4.55

5.01

 MLCK

6.28

4.03

Cell migration and adhesion

 Integrin β1

5.10

3.71

 Integrin α2

5.66

4.89

 Myosin IA

4.27

6.37

 Myosin IE

4.44

8.35

 Myosin VIIA

4.31

11.90

 Nonmuscle myosin II

3.75

4.07

 N-cadherin (neuronal)

3.79

6.35

 VE-cadherin (vascular)

2.90

5.76

 Matrix metallopeptidase 7

3.41

3.55

 Matrix metallopeptidase 10

2.94

2.26

 TIMP metallopeptidase inhibitor 3

0.40

0.33

 E-cadherin (epithelial)

0.22

0.25

  1. Tumour cell mRNA was transcripted into cDNA and labeled with either Cy3 or Cy5. The activity of genes involved in cell migration/cell adhesion, in proliferation/cell cycle, and in signal transduction was investigated. The DNA microarray analysis was performed three times and only data with higher than 2.5-fold changes in gene activity were taken into consideration.
Back to article page