Figure 2

Expression of KIF20A mRNA and protein in human normal tissues, cancer cell lines, and pancreatic cancer tissues. (A) RT–PCR analysis of KIF20A mRNA expression in various normal tissues. KIF20A mRNA was not detected except for faint expression observed in the testis. (B) The expression of KIF20A mRNA and protein in various cancer cell lines investigated by RT–PCR (left) and western blot analyses (right). KIF20A mRNA was detected in all pancreatic cancer cell lines examined. The major bands at ∼100 kDa, which corresponds to the calculated molecular weight for KIF20A, are shown. It must be noted that SKHep1 and SKHep1/Mock expressed only a trace amount of the KIF20A protein, whereas KIF20A cDNA-transfected SKHep1, SKHep1/KIF20A, relatively expressed a large amount of the protein. (C) RT–PCR analyses of the KIF20A expression in pancreatic cancer tissues (T), and their normal counterparts (N). The expression of the KIF20A mRNA was detected in five of eight pancreatic tumour tissues, but little expression was detected in their normal counterparts. It must be noted that KIF20A mRNA expression was also detected in the peritoneum (patient 9 (Pt9)) and metastatic foci of the skin (patient 10 (Pt10)).