Table 1 S1 and S4 gene mutations of persistently infecting reoviruses

From: Attenuated reovirus displays oncolysis with reduced host toxicity

  

Mutation position

 

Persistent reovirus

Reovirus gene

Nucleotide

Amino acid

GenBank accession no.

HTR1

S1

359, T–C

116, L–P

AY860061

  

392, T–C

127, V–A

 
  

763, C–T

251, Q–Stop

 
  

912, A–G

300, I–M

 
 

S4

562, C–T

177, S–F

DQ915165

  

784, A–T

251, H–L

 

CA46 PI

S1

89, A–C

26, K–T

EF133509

   

7, K–Q

 
  

1351, A–T

447, T–S

 
 

S4

720, C–T

230, H–Y

EF133510

  

730, C–T

233, S–L

 
  

1089, A–C

353, N–Q

 
  

1091, T–G

353, N–Q

 
  

1092, T–C

354, Y–H

 

Raji PI

S1

—

—

EF133511

 

S4

784, A–T

251, H–L

EF133512

  1. Mutations in S1 and S4 gene nucleotide sequences of persistently infecting reoviruses from HTR1, Raji PI and CA46 PI cells, and corresponding mutations in the deduced amino acid sequences of their gene products (S1: σ1/σ1 s, S4: σ3) compared with previously published sequences of WT reovirus (GenBank accession no: S1 (X01161), S4 (K02739)). As reovirus occasionally undergoes spontaneous genome variations, nucleotide changes that were found in all the persistently infecting reoviruses and in our lab, WT reovirus were not included in this table (S1: 366, C–G, 367, G–C, S4: 624, G–A, 719, G–T).
Back to article page