Table 1 Altered expression of nuclear receptors in brain tumour stem cells

From: Constitutive androstane receptor agonist CITCO inhibits growth and expansion of brain tumour stem cells

Receptor

Fold change

Receptor

Fold change

ERRβ (NR3B2)

ND-D

TR4 (NR2C2)

0.995

RXRÎł (NR2B3)

ND-D

TRα (NR1A1)

0.965

TLX (NR2E1)

628.610

ERRα (NR3B1)

0.934

HNF4α (NR2A1)

272.000

TR2 (NR2C1)

0.890

PXR (NR1I2)

27.800

RXRα (NR2B1)

0.848

RORα (NR1F1)

19.690

EAR (NR2F6)

0.706

RORÎł (NR1F3)

18.280

PPARÎł (NR1C3)

0.648

NORI (NR4A3)

11.426

Coup TF2 (NR2F2)

0.605

LRH (NR5A2)

10.480

RARÎł (NR1B3)

0.565

NURR1 (NR4A2)

7.040

TRβ (NR1A2)

0.511

NURR77 (NR4A1)

4.380

AR (NR3C4)

0.478

REVerbα (NR1D1)

2.960

RARβ (NR1B2)

0.425

MR (NR3C2)

2.507

HNF4Îł (NR2A2)

0.353

RARα (NR1B1)

2.260

Coup TF1 (NR2F1)

0.332

LXRβ (NR1H2)

2.051

CAR (NR1I3)

0.112

PPARδ (NR1C2)

1.652

FXR (NR1H4)

0.104

GR (NR3C1)

1.600

RORβ (NR1F2)

0.080

PPARα (NR1C1)

1.421

SF1 (NR5A1)

0.0789

PGR (NR3C3)

1.330

PNR (NR2E3)

0.00023

VDR (NR1I1)

1.248

ERα (NR3A1)

ND-ND

RXRβ (NR2B2)

1.195

ERβ (NR3A2)

ND-ND

GCNF (NR6A1)

1.126

ERRÎł (NRB3)

ND-ND

REVerbβ (NR1D2)

1.150

DAX (NR0B1)

ND-ND

LXRα (NR1H3)

1.089

SHP (NR0B2)

ND-ND

  1. Abbreviations: bFGF=basic fibroblast growth factor; BTSC=brain tumour stem cell; cDNA=complementary DNA; DMEM=Dulbecco's modified Eagle's medium; EGF=epidermal growth factor; FBS=fetal bovine serum; NBM=neurobasal medium.
  2. The T98G glioma cells were cultured as monolayer in DMEM with 10% FBS or as gliospheres in NBM with B27 and EGF+bFGF. After 5 days, the glioma cells were harvested using trypsin+EDTA, and gliospheres were dissociated using Accutase. Total RNA was extracted from glioma and BTSCs, and reverse transcribed into cDNA using random hexamer primers and TaqMan reverse transcription kit. The gene expression profile was determined using TaqMan Universal Master Mix with nuclear receptor primer sets and probes in an optical 384-well card using the 7900 Fast Sequence Detection Real-time PCR System (Applied Biosystems). The results were analysed using the Prism 7900 relative quantification (delta delta Ct) study software. The levels of nuclear receptor gene expression are normalised to 18 s, and the values are presented as fold change in BTSCs compared with glioma cells.
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