Figure 3 | British Journal of Cancer

Figure 3

From: Human prostate cell lines from normal and tumourigenic epithelia differ in the pattern and control of choline lipid headgroups released into the medium on stimulation of protein kinase C

Figure 3

PNT2C2, PNT1A, P4E6, LNCaP and PC3 prostate cell lines show basal and TPA-stimulated PLD activity in the transphosphatidylation reaction. Cells were stimulated for 30 min with 4α-phorbol to measure basal (B) PLD activity, 1 μ M TPA (T) or 1 μ M TPA+1 μ M Ro31-8220 (TR). Lipids were extracted and resolved as described in Materials and Methods. Formation of PtdBut is expressed as % PtdBut d.p.m./PtdCho d.p.m. Results are ±s.e.m., n=3. ***P<0.0001 compared with basal and TPA/Ro31-8220-treated cells.

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