Figure 4

Prostate cell lines do not release [3H]-label into the medium as vesicles. Levels of radioactivity released into the medium from PNT2C2, PNT1A, P4E6 and PC3 cells did not decrease on low- or high-speed centrifugation as described. Results shown are for medium from cells stimulated with TPA for 3 h before centrifugation (1) and after low-speed (2) and high-speed (3) and are ±s.d., n=3.