Figure 6 | British Journal of Cancer

Figure 6

From: Human prostate cell lines from normal and tumourigenic epithelia differ in the pattern and control of choline lipid headgroups released into the medium on stimulation of protein kinase C

Figure 6

(A) Protein kinase C inhibitors reduce phorbol ester-stimulated release of [3H] label into the medium from PNT2C2 and PNT1A cells. PNT2C2, PNT1A, LNCaP and PC3 cell lines labelled with [3H]-choline were stimulated as described in Materials and Methods section with 1 μ M 4α-phorbol (basal, B), 1 μ M TPA (TP), 1 μ M TPA+1 μ M Ro31-8220 (Ro), 1 μ M TPA+1 μ M GF-109203X (GF) or 1 μ M TPA+1 μ M Go6976 (Go) and release of [3H]-label into the medium was monitored at 3 h. In both PNT2C2 and PNT1A cells, PKC inhibitors reduced release to basal values. Results are ±s.d. (n=6). For PNT2C2 and PNT1A cells, *P<0.01, **P<0.001, ***P<0.0001 against the basal value. For LNCaP cells **P< 0.01 against the basal value. (B) D609 and hemicholinium-3 effects on [3H]-label release by prostate cell lines. PNT2C2, PNT1A, P4E6, LNCaP and PC3 cells labelled with [3H]-choline were stimulated as described in Materials and Methods with 1 μ M 4α-phorbol (basal, B), 1 μ M TPA (T), 1 μ M TPA+200 μ M HC-3 (H) or 1 μ M TPA+100 μ M D609 (D) and release of [3H]-choline metabolites to the medium was monitored at 3 h. Results are ±s.d. (n=6). For PNT2C2 cells ***P<0.0001 against the basal value. For PNT1A cells **P<0.001 against the TPA value, ***P<0.0001 against basal value. For LNCaP *P<0.05 against the basal value, **P<0.01 against the TPA value.

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