Summary
High fat diets have been implicated in incidence of colon cancer both in epidemiological and animal studies. Present investigation deals with the incidence, location and numbers of large and small bowel tumours induced by 1,2-dimethyl hydrazine (DMH) in rats fed high fat diets and neomycin. Neomycin was used to modify the faecal sterol metabolism and the relationship of the high fat diet and faecal neutral and acid sterols to the large bowel tumorigenesis was evaluated. DMH administered rats were fed with (a) 20% safflower oil; (b) 20% safflower oil and neomycin; (c) 20% safflower oil, cholesterol and cholic acid; and (d) 20% safflower oil, cholesterol, cholic acid and neomycin. Neomycin was found to be associated with both increase and decrease of tumour numbers. The faecal sterols lithocholic and deoxycholic acids were found to have no participation, while cholesterol and cholic acid were found to decrease with increase in tumour numbers. However, faecal coprostanol has been found to have a significant positive correlation with tumorigenesis in all dietary groups. Therefore coprostanol might possibly be associated with colon carcinogenesis in DMH-fed rats and cholesterol metabolism in gut appears to be related to the development of tumours.
Similar content being viewed by others
Article PDF
Change history
16 November 2011
This paper was modified 12 months after initial publication to switch to Creative Commons licence terms, as noted at publication
References
Broitman, S. A., Kinnear, D. G., Gottlieb, L. S., Bezman, A. L., Vitale, J. J. & Zamcheck, N. (1960). Effect of neomycin alteration on the rat intestinal flora on serum cholesterol and valvular sudanophilia. J Lab Clin Med 55: 55–59.
Broitman, S. A., Vitale, J. J., Vavrousek-Jakuba, E. & Gottlieb, L. S. (1977). Polyunsaturated fat, cholesterol and large bowel tumorigenesis. Cancer 40: 2455–2463.
Cannizzo, F. Jr & Broitman, S. A. (1989). Postpromotional effects of dietary marine or safflower oils on large bowel or pulmonary implants of CT-26 in mice. Cancer Res 49: 4289–4294.
Carpenter, K., Gotsis, A. & Hegsted, D. M. (1957). Estimation of total cholesterol in serum. Clin Chem 3: 233–288.
Carroll, K. K. (1987). Summation: which fat/how much fat – animals. Prev Med 16: 510–515.
Cerda, S. R., Wilkinson, J. I. V. & Broitman, S. A. (1995). Regulation of cholesterol synthesis in four colonic adenocarcinoma cell lines. Lipids 30: 1083–1092.
Cohen, B. I., Raicht, R. F., Deschner, E. E., Fazzini, E., Takahashi, M. & Sarwal, A. (1978). Effects of bile acids on induced colon cancer in rats. Proc Am Assoc Cancer Res 19: 48
Corpet, D. E., Bellier, R., Petrowitsch, S. & Vigouroux, Y. (1994). Digestion and fermentation of proteins in rats fed keratin, albumin, cooked casein and antibiotics. Reprod Nutr Dev 34: 57–64.
Deschner, E. E., Winawer, S. J., Long, F. C. & Boyle, C. C. (1977). Early detection of colonic neoplasia in patients at high risk. Cancer 40: 2625–2631.
Doll, R. (1969). The geographical distribution of cancer. Br J Cancer 23: 1–8.
Fabricant, M. & Broitman, S. A. (1990). Evidence for deficiency of low density lipoprotein receptor on human colonic carcinoma cell lines. Cancer Res 50: 632–636.
Grundy, S. M., Ahrens, E. H. & Miettinen, T. A. (1965). Quantitative isolation and gas-liquid chromatographic analysis of the total bile acids. J Lipid Res 6: 397–410.
Haenszel, W., Berg, J. W., Kurihara, M. & Locke, F. B. (1973). Large bowel cancer in Hawaiian Japanese. J Natl Cancer Inst 51: 1965–1799.
Kesaniemi, Y. A. & Miettinen, T. A. (1991). Inhibition of cholesterol absorption by neomycin, benzodiazepine derivatives and ketoconazole. Eur J Clin Pharmacol 40: S65–S67.
Kraus, L. J., Williams, R. M. & Broitman, S. A. (1987). T-cell mitogenesis and natural killer cell activity in colonic tumor-bearing and nontumor-bearing rats fed diets high in lipid with and without cholesterol. Nutr Cancer 9: 159–170.
Lindner, M. A. (1991). A fish oil diet inhibits colon cancer in mice. Nutr Cancer 15: 1–11.
Miettinen, T. A., Ahrens, E. H. & Grundy, S. M. (1965). Quantitative isolation and gas-liquid chromatographic analysis of total dietary and faecal neutral steroids. J Lipid Res 6: 411–424.
Narisawa, T., Takahashi, M., Kotanagi, H., Kusaka, H., Yamazaki, Y., Koyama, H., Fukaura, Y., Nishizawa, Y., Kotsugai, M. & Isoda, Y. (1991). Inhibitory effect of dietary perilla oil rich in the n-3 polyunsaturated fatty acid alpha-linolenic acid on colon carcinogenesis in rats. Jpn J Cancer Res 82: 1089–1096.
Narisawa, T., Fukaura, Y., Yazawa, K., Ishikawa, C., Isoda, Y. & Nishizawa, Y. (1994). Colon cancer prevention with a small amount of dietary perilla oil high in alpha-linolenic acid in an animal model. Cancer 73: 2069–2075.
Ochiai, M., Nakagama, H., Watanabe, M., Ishiguro, Y., Sugimura, T. & Nagao, M. (1996). Efficient method for rapid induction of aberrant crypt foci in rats with 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine. Jpn J Cancer Res 87: 1029–1033.
Raicht, R. F., Cohen, B. I., Shefer, S. & Mosbach, E. H. (1975). Sterol metabolism in the rat. Effect of dietary cholesterol and β-sitosterol and sterol balance and rate limiting enzymes of sterol metabolism. Biochim Biophys Acta 388: 374–384.
Reddy, B. S. (1992). Dietary fat and colon cancer: animal model studies. Lipids 27: 807–813.
Reddy, B. S., Martin, C. W. & Wynder, E. L. (1977). Faecal bile acids and cholesterol metabolites of patients with ulcerative colitis, a high risk group for development of colon cancer. Cancer Res 37: 1697–1701.
Sedaghat, A., Samuel, P., Crouse, J. R. & Ahrens, E. H. (1975). Effects of neomycin on absorption, synthesis and/or flux of cholesterol in man. J Clin Invest 55: 12–21.
Steiner, A., Weisser, B. & Vetter, W. (1991). A comparative review of the adverse effects of treatments for hyperlipidaemia. Drug Saf 6: 118–130.
Wynder, E. L. (1975). The epidemiology of large bowel cancer. Cancer Res 35: 338–3394.
Author information
Authors and Affiliations
Rights and permissions
From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
About this article
Cite this article
Panda, S., Chattoraj, S. & Broitman, S. Correlation of neomycin, faecal neutral and acid sterols with colon carcinogenesis in rats. Br J Cancer 80, 1132–1136 (1999). https://doi.org/10.1038/sj.bjc.6690476
Received:
Revised:
Accepted:
Published:
Issue date:
DOI: https://doi.org/10.1038/sj.bjc.6690476
