Abstract
Differential gene expression between squamous cell carcinoma of the head and neck and matched normal tissue was studied by utilizing Representational Difference Analysis. Using this methodology, a novel gene, DESC1 was isolated. DESC1 possesses strong identity to the serine protease super-family. Comparison of DESC1 expression between primary squamous cell carcinoma and matched normal tissue shows that the level of DESC1 expression is reduced or absent in 11/12 SCC tissue specimens when compared to specimens of matched normal tissue. Tissue-specific expression studies further show that DESC1 expression can only be detected in tissues derived from the head and neck, and in skin, prostate and testes. Cell line studies demonstrate that DESC1 expression is epithelial-specific. Chromosomal localization studies indicate that DESC1 is located on the long arm of chromosome 4 at position q12–13. © 2001 Cancer Research Campaign http://www.bjcancer.com
Similar content being viewed by others
Article PDF
Change history
16 November 2011
This paper was modified 12 months after initial publication to switch to Creative Commons licence terms, as noted at publication
References
Bockmuhl U, Wolf G, Schmidt S, Schwendel A, Jahnke V, Dietel M and Peterson I (1998) Genomic alterations associated with malignancy in head and neck cancer. Head Neck 20: 145–151
Califano J, Van Der Riet P, Westra W, Nawroz H, Clayman G, Piantadosi S, Corio R, Lee D, Greenberg B, Koch W and Sidransky D (1996) Genetic progression model for head and neck cancer: implications for field cancerization. Cancer Res 56: 2488–2492
Cheah K-C, Leong LE-C and Porter AG (1990) Site directed mutagenesis suggests close functional relationship between a human rhinovirus 3C cysteine protease and cellular trypsin-like serine proteases. J Biol Chem 265: 7180–7187
Diatchenko L, Lau YFC, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED and Siebert PD (1996) Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci USA 93: 6025–6030
Field JK, Spandidos DA, Stell PM, Vaughan ED, Evan GI and Moore JP (1989) Elevated expression of the c-myc oncoprotein correlates with poor prognosis in head and neck squamous cell carcinoma. Oncogene 4: 1463–1468
Goyal J, Smith KM, Cowan JM, Wazer DE, Lee SW and Band V (1998) The role for NES1 serine protease as a novel tumor suppressor. Cancer Res 58: 4782–4786
Gramza AW, Lucas JM, Mountain RE, Schuller DE and Lang JC (1995) Efficient method for preparing normal and tumor tissue for RNA extraction. Biotechniques 18: 228
Ke LD, Adler-Storthz K, Clayman GL, Yung AW and Chen Z (1998) Differential expression of epidermal growth factor receptor in human head and neck cancers. Head Neck 20: 320–327
Lang JC, Tobin EJ, Knobloch TJ, Bartynski KJ, Mountain RE, Nicholson R, De Young BR, Weghorst CM and Schuller DE (1998) Frequent mutation of p16 in squamous cell carcinoma of the head and neck. Laryngoscope 108: 923–928
Liotta LA and Stetler-Stevenson WG (1991) Tumor invasion and metastasis: An imbalance of positive and negative regulation. Cancer Res 51: 5054–5059
Loughran O, Clark LJ, Bond J, Baker A, Berry IJ, Edington KG, Ly IS, Simmons R, Haw R, Black DM, Newbold RF and Parkinson EK (1997) Evidence for the inactivation of multiple replicative lifespan genes in immortal human squamous cell carcinoma keratinocytes. Oncogene 14: 1955–1964
Michalides R, Van Veelen N, Hart A, Loftus B, Wientjens E and Balm A (1995) Overexpression of cyclin D1 correlates with recurrence in a group of forty-seven operable squamous cell carcinomas of the head and neck. Cancer Res 55: 975–978
Nawroz H, van der Riet P, Hruban RH, Koch W, Ruppert JM and Sidransky D (1994) Allelotype of head and neck squamous cell carcinoma. Cancer Res 54: 1152–1155
Reed AL, Califano J, Cairns P, Westra WH, Jones RM, Koch W, Ahrendt S, Eby Y, Sewell D, Nawroz H, Bartek J and Sidransky D (1996) High frequency of p16 (CDKN2/MTS-1/INK4A) inactivation in head and neck squamous cell carcinoma. Cancer Res 56: 3630–3633
Somers KD, Merrick MA, Lopez ME, Incognito LS, Schechter GL and Casey G (1992) Frequent p53 mutations in head and neck cancer. Cancer Res 52: 5997–6000
Xia W, Lau YK, Zhang HZ, Liu AR, Li L, Kiyokawa N, Clayman GL, Katz RL and Hung MC (1997) Strong correlation between c-erbB-2 overexpression and overall survival of patients with oral squamous cell carcinoma. Clin Can Res 3: 3–9
Yamaoka K, Masuda K, Ogawa H, Takagi K, Umemoto N and Yasuoka S (1998) Cloning and characterization of the cDNA for human airway trypsin-like protease. J Biol Chem 273: 11895–11901
Author information
Authors and Affiliations
Rights and permissions
From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
About this article
Cite this article
Lang, J., Schuller, D. Differential expression of a novel serine protease homologue in squamous cell carcinoma of the head and neck. Br J Cancer 84, 237–243 (2001). https://doi.org/10.1054/bjoc.2000.1586
Received:
Revised:
Accepted:
Published:
Issue date:
DOI: https://doi.org/10.1054/bjoc.2000.1586
Keywords
This article is cited by
-
An investigation of the relationship between TMPRSS6 gene expression, genetic variants and clinical findings in breast cancer
Molecular Biology Reports (2020)
-
Cell surface–anchored serine proteases in cancer progression and metastasis
Cancer and Metastasis Reviews (2019)
-
Matriptase-2 inhibits HECV motility and tubule formation in vitro and tumour angiogenesis in vivo
Molecular and Cellular Biochemistry (2012)
-
Human DESC1 serine protease confers tumorigenic properties to MDCK cells and it is upregulated in tumours of different origin
British Journal of Cancer (2007)
