Abstract
We assessed the relative expression of oestrogen receptor (ER)α and oestrogen receptor (ER)β mRNAs in 36 human endometrial cancers using a multiplex polymerase chain reaction (PCR). To determine whether or not the expression of ER subtypes in endometrial cancers is associated with clinicopathological parameters, we examined correlations between ER subtypes and age, tumour grade and depth of myometrial invasion. Using multiple regression analysis, myometrial invasion showed a significant correlation with ER-β: ER-α ratio (r = 0.54, P = 0.0007). The ER-β:ER-α ratio was high in advanced invasive carcinoma. Western blotting analysis showed that ER-β proteins were highly expressed in comparison with ER-α proteins in endometrial cancer with severe myometrial invasion. Our results suggest that ER-β is important in the progression of myometrial invasion. http://www.bjcancer.com © 2001 Cancer Research Campaign
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References
Berliere M, Charles A, Galant C and Donnez J (1998) Uterine side effects of tamoxifen: a need for systematic pretreatment screening. Obstet Gynecol 91: 40–44
Dotzlaw H, Leygue E, Watson P and Murphy L (1997) Expression of estrogen receptor-β in human breast tumors. J Clin Endocrinol Metab 82: 2371–2374
Dotzlaw H, Leygue E, Watson P and Murphy LC (1999) Estrogen receptor-β messenger RNA expression in human breast tumour biopsies: relationship to steroid receptor status and regulation by progestins. Cancer Res 59: 529–532
Friberg LG and Noren H (1993) Prognostic value of steroid hormone receptors for 5-year survival in stage II endometrial cancer. Cancer 71: 3570–3574
Gaub MP, Bellard M, Scheuer I, Chambon P and Sassone CP (1990) Activation of the ovalbumin gene by the estrogen receptor involves the fos-jun complex. Cell 63: 1267–1276
Hyder SM, Stancel GM, Chiapetta C, Murthy L, Boettger THL and Makela S (1996) Uterine expression of vascular endothelial growth factor is increased by estradiol and tamoxifen. Cancer Res 56: 3954–3960
Iwai K, Fukuda K, Hachisuga T, Mori M, Uchiyama M, Iwasaka T and Sugimori H (1999) Prognostic significance of progesterone receptor immunohistochemistry for lymph node metastases in endometrial carcinoma. Gynecol Oncol 72: 351–359
Kadar N, Malfetano JH and Homesley HD (1993) Steroid receptor concentrations in endometrial carcinoma: effect on survival in surgically staged patients. Gynecol Oncol 50: 281–286
Kuiper GGJM, Enmark E, Pelto-Huikko M, Nilsson S and Gustafsson JA (1996) Cloning of a novel estrogen receptor expressed in rat prostate and ovary. Proc Natl Acad Sci USA 93: 5925–5930
Kuiper GGJM, Carlsson B, Grandien K, Enmark E, Haggblad J, Nilsson S and Gustafsson JA (1997) Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinology 183: 863–870
La Vecchia C, Franceschi S, Parazzini F Colombo E, Colombo F, Liberat A and Mangioni C (1983) Ten-year survival in 290 patients with endometrial cancer: prognostic factors and therapeutic approach. Br J Obstet Gynecol 90: 654–661
Leygue E, Dotzlaw H, Watson P and Murphy L (1998) Altered estrogen receptor α and β mRNA expression during human breast tumorigenesis. Cancer Res 58: 3197–3201
Lu B, Leygue E, Dotzlaw H, Murphy LJ, Murphy LC and Watson PH (1998) Estrogen receptor-β mRNA variants in human and murine tissues. Mol Cell Endocrinol 138: 199–203
Morris PC, Anderson JR, Anderson B and Bulle RE (1995) Steroid hormone receptor content and lymph node status in endometrial cancer. Gynecol Oncol 56: 406–411
Morrow CP, Bundy BN, Kurman RJ, Creasman WT, Heller P, Homesley HD and Graham JE (1991) Relationship between surgical-pathological risk factors and outcome in clinical stage I and II carcinoma of the endometrium. A Gynecologic Oncology Group Study. Gynecol Oncol 40: 55–65
Mosselman S, Polman J and Dijkema R (1996) ER-β: identification and characterization of a novel estrogen receptor. FEBS Lett 392: 49–53
Moutsatsou P and Sekeris CE (1997) Estrogen and progesterone receptors in the endometrium. Ann N-Y-Acad Sci 816: 99–115
Osborne CK (1998) Tamoxifen in the treatment of breast cancer. N Engl J Med 339: 1609–1618
Paech K, Webb P, Kuiper GGJM, Nilsson S, Gustaffson JA, Kushner PJ and Scanlan TS (1998) Differential ligand activation of estrogen receptors ERα and ERβ at AP-1 sites. Science 277: 1508–1510
Speirs V, Parkes Alicia T, Kerin MJ, Walton DS, Carleton PJ, Fox JN and Atkin SL (1999a) Coexpression of estrogen receptor α and β: poor prognostic factors in human breast cancer?. Cancer Res 59: 525–528
Speirs V, Malone C, Walton DS, Kerin MJ and Atkin SL (1999b) Increased expression of estrogen receptor beta mRNA in tamoxifen-resistant breast cancer patients. Cancer Res 59: 5421–5424
Tremblay GB, Tremblay A, Copeland NG, Gilbert D, Jenkins NA, Labrie F and Giguere V (1997) Cloning, chromosomal localization, and functional analysis of the murine estrogen receptor β. Mol Endocrinol 11: 353–365
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Takama, F., Kanuma, T., Wang, D. et al. Oestrogen receptor β expression and depth of myometrial invasion in human endometrial cancer. Br J Cancer 84, 545–549 (2001). https://doi.org/10.1054/bjoc.2000.1589
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DOI: https://doi.org/10.1054/bjoc.2000.1589
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