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Identification of human renal cell carcinoma associated genes by suppression subtractive hybridization
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  • Published: 30 October 2001

Identification of human renal cell carcinoma associated genes by suppression subtractive hybridization

  • M J J G Stassar1 nAff8,
  • G Devitt1,
  • M Brosius1,
  • L Rinnab2,
  • J Prang2,
  • T Schradin2,
  • J Simon2,
  • S Petersen3 nAff9,
  • A Kopp-Schneider4 &
  • …
  • M Zöller1,5 

British Journal of Cancer volume 85, pages 1372–1382 (2001)Cite this article

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Abstract

Renal cell carcinoma (RCC) are frequently chemo- and radiation resistant. Thus, there is a need for identifying biological features of these cells that could serve as alternative therapeutic targets. We performed suppression subtractive hybridization (SSH) on patient-matched normal renal and RCC tissue to identify variably regulated genes. 11 genes were strongly up-regulated or selectively expressed in more than one RCC tissue or cell line. Screening of filters containing cancer-related cDNAs confirmed overexpression of 3 of these genes and 3 additional genes were identified. These 14 differentially expressed genes, only 6 of which have previously been associated with RCC, are related to tumour growth/survival (EGFR, cyclin D1, insulin-like growth factor-binding protein-1 and a MLRQ sub-unit homologue of the NADH:ubiquinone oxidoreductase complex), angiogenesis (vascular endothelial growth factor, endothelial PAS domain protein-1, ceruloplasmin, angiopoietin-related protein 2) and cell adhesion/motility (protocadherin 2, cadherin 6, autotaxin, vimentin, lysyl oxidase and semaphorin G). Since some of these genes were overexpressed in 80–90% of RCC tissues, it is important to evaluate their suitability as therapeutic targets. © 2001 Cancer Research Campaign www.bjcancer.com

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  • 16 November 2011

    This paper was modified 12 months after initial publication to switch to Creative Commons licence terms, as noted at publication

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Author notes

  1. M J J G Stassar

    Present address: Recombinant Antibody Group, German Cancer Research Center, 69120, Heidelberg, Germany

  2. S Petersen

    Present address: Exp. Immunology Branch, NCI / NIH, 10 Center Drive, Bethesda, MD, 20892, USA

Authors and Affiliations

  1. Department of Tumor Progression and Immune Defense, German Cancer Research Center, Heidelberg, 69120

    M J J G Stassar, G Devitt, M Brosius & M Zöller

  2. Department of Urology, University Hospital, Ulm, 89075

    L Rinnab, J Prang, T Schradin & J Simon

  3. Institute of Pathology, University Hospital Charité, Berlin, 10098

    S Petersen

  4. Department of Biostatistics, German Cancer Research Center, Heidelberg, 69120

    A Kopp-Schneider

  5. Department of Applied Genetics, University of Karlsruhe, Karlsruhe, 76128, Germany

    M Zöller

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Stassar, M., Devitt, G., Brosius, M. et al. Identification of human renal cell carcinoma associated genes by suppression subtractive hybridization. Br J Cancer 85, 1372–1382 (2001). https://doi.org/10.1054/bjoc.2001.2074

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  • Received: 25 January 2001

  • Revised: 18 July 2001

  • Accepted: 24 July 2001

  • Published: 30 October 2001

  • Issue date: 02 November 2001

  • DOI: https://doi.org/10.1054/bjoc.2001.2074

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Keywords

  • human
  • renal cell carcinoma
  • gene expression

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