Table 1 Sequence divergence among multiple PCR products of a given primer pair based on the substitution model of Hasegawa et al (1985)

Locus	bp analysed	Number of distinct sequences ^a	Range of fragment lengths	Min. divergence	Max. divergence	Min. divergence (b-v)
Bb41.11^b	60	11	247–811	0.018	0.054	0.018
Bb42.22^b	185	3	237–580	0.076	0.109	0.076
Bb41.4	102	21	187–201	0	0.113	0
Bb1.1	204	5	249–273	0.034	0.138	0.034
Bb1.20	168	6	249–281	0.006	0.743	0.083
Bb3.17	201	8	266–277	0.010	0.113	0.010
Bb1.4	199	7	247–265	0.005	0.558	0.005
Bv24.11	123	3	168	0.016	0.016	0.016

All comparisons include the originally sequenced clone. Alignment gaps, microsatellites and primers were excluded from the analysis. Listed are the minimum and maximum divergences for a given primer pair irrespective of taxon or fragment length and the minimum divergence for any bombina variegata pair of PCR products.
^aNumbers represent the sum of separate B. bombina and B. variegata lists of distinct sequences.
^bHomologous sequence stretches only.

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