Table 2 cpDNA regions sequenced to search for polymorphic microsatellite loci in S. romanzoffiana

From: Population genetic structure in European populations of Spiranthes romanzoffiana set in the context of other genetic studies on orchids

Chloroplast region

Annealing temp. (°C)

Extension time (min)

Primer source

GenBank accession no.

atpB-rbcL

53

3

Chiang et al (1998)

AY363058, AY363059

trnL-intron-trnL-trnF IGS

55

3

Taberlet et al (1991)

AY363055

psbC-trnS

57

3

Demesure et al (1995)

AY363056, AY363057

trnS-trnfM

62

3

Demesure et al (1995)

AY363054

trnH-trnK

62

3

Demesure et al (1995)

AY363060, AY363061