Table 5 Number of individuals scored (n), number of alleles (A), mean number of alleles (MNA), expected heterozygosity (HE), observed heterozygosity (HO) and FIS according to Weir and Cockeram (1984) for the microsatellite loci
Locus | Samples | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
K1 | K12 | Zs | Ra2 | Ot1 | Ot2 | Np | Tx | Of1 | W1 | W2 | Oc7 | Oc15 | Oc21 | Oc37 | |
Pmin-01 | |||||||||||||||
n | 56 | 53 | 49 | 52 | 53 | 48 | 49 | 49 | 36 | 52 | 47 | 50 | 50 | 41 | 52 |
A | 45 | 33 | 34 | 37 | 49 | 39 | 43 | 46 | 18 | 49 | 46 | 46 | 38 | 25 | 46 |
HE | 0.967 | 0.939 | 0.953 | 0.954 | 0.971 | 0.958 | 0.965 | 0.967 | 0.940 | 0.968 | 0.963 | 0.966 | 0.962 | 0.936 | 0.970 |
HO | 0.696 | 0.623 | 0.674 | 0.750 | 0.868 | 0.834 | 0.898 | 0.776 | 0.833 | 0.808 | 0.787 | 0.860 | 0.960 | 0.927 | 0.904 |
FIS | 0.288 | 0.345 | 0.303 | 0.223 | 0.116 | 0.141 | 0.08 | 0.208 | 0.105 | 0.175 | 0.193 | 0.120 | 0.012 | 0.022 | 0.079 |
Pmin-05 | |||||||||||||||
n | 57 | 50 | 52 | 53 | 54 | 51 | 50 | 51 | 36 | 54 | 51 | 49 | 49 | 43 | 52 |
A | 56 | 37 | 32 | 39 | 44 | 33 | 40 | 39 | 22 | 46 | 38 | 31 | 40 | 39 | 26 |
HE | 0.976 | 0.956 | 0.947 | 0.962 | 0.966 | 0.959 | 0.962 | 0.964 | 0.940 | 0.967 | 0.964 | 0.950 | 0.964 | 0.961 | 0.942 |
HO | 0.860 | 0.660 | 0.635 | 0.755 | 0.926 | 0.843 | 0.860 | 0.765 | 0.889 | 0.815 | 0.843 | 0.714 | 0.755 | 0.977 | 0.689 |
FIS | 0.128 | 0.319 | 0.338 | 0.224 | 0.051 | 0.131 | 0.116 | 0.216 | 0.069 | 0.166 | 0.135 | 0.258 | 0.227 | −0.004 | 0.279 |
Pmin-06 | |||||||||||||||
n | 57 | 52 | 53 | 53 | 54 | 51 | 51 | 49 | 36 | 53 | 52 | 52 | 48 | 44 | 49 |
A | 29 | 27 | 23 | 25 | 22 | 26 | 20 | 24 | 19 | 27 | 24 | 13 | 13 | 24 | 30 |
HE | 0.911 | 0.914 | 0.919 | 0.925 | 0.909 | 0.930 | 0.904 | 0.920 | 0.882 | 0.886 | 0.871 | 0.723 | 0.833 | 0.902 | 0.863 |
HO | 0.860 | 0.679 | 0.736 | 0774 | 0.722 | 0.846 | 0.843 | 0.612 | 0.750 | 0.887 | 0.904 | 0.750 | 0.750 | 0.818 | 0.694 |
FIS | 0.065 | 0.266 | 0.208 | 0.173 | 0.214 | 0.100 | 0.077 | 0.344 | 0.164 | 0.009 | −0.028 | −0.028 | 0.109 | 0.104 | 0.205 |
Pmin-07 | |||||||||||||||
n | 57 | 53 | 52 | 53 | 54 | 50 | 47 | 46 | 36 | 53 | 50 | 52 | 51 | 43 | 52 |
A | 6 | 6 | 6 | 6 | 5 | 5 | 5 | 7 | 3 | 7 | 5 | 6 | 6 | 6 | 6 |
HE | 0.697 | 0.702 | 0.607 | 0.700 | 0.652 | 0.634 | 0.607 | 0.764 | 0.538 | 0.704 | 0.607 | 0.691 | 0.641 | 0.499 | 0.606 |
HO | 0.386 | 0.660 | 0.442 | 0.697 | 0.611 | 0.680 | 0.500 | 0.717 | 0.679 | 0.453 | 0.500 | 0.500 | 0.451 | 0.465 | 0.673 |
FIS | 0.453 | 0.069 | 0.280 | 0.039 | 0.072 | −0.069 | 0.207 | 0.072 | 0.239 | 0.365 | 0.186 | 0.286 | 0.305 | 0.079 | −0.102 |
Pmin-08 | |||||||||||||||
n | 56 | 52 | 53 | 49 | 52 | 51 | 46 | 47 | 36 | 54 | 50 | 48 | 48 | 45 | 52 |
A | 29 | 28 | 25 | 25 | 22 | 26 | 26 | 25 | 20 | 23 | 24 | 21 | 30 | 27 | 29 |
HE | 0.936 | 0.894 | 0.900 | 0.924 | 0.879 | 0.913 | 0.914 | 0.928 | 0.888 | 0.926 | 0.918 | 0.923 | 0.946 | 0.930 | 0.940 |
HO | 0.857 | 0.884 | 0.849 | 0.816 | 0.692 | 0.922 | 0.804 | 0.872 | 0.889 | 0.833 | 0.900 | 0.771 | 0.792 | 0.910 | 0.810 |
FIS | 0.094 | 0.020 | 0.064 | 0.127 | 0.221 | 0.001 | 0.131 | 0.070 | 0.013 | 0.110 | 0.030 | 0.175 | 0.174 | 0.031 | 0.149 |
Pmin-10 | |||||||||||||||
n | 58 | 53 | 53 | 53 | 54 | 52 | 50 | 51 | 36 | 53 | 50 | 52 | 50 | 45 | 52 |
A | 28 | 32 | 14 | 26 | 24 | 26 | 22 | 21 | 19 | 28 | 28 | 18 | 22 | 30 | 24 |
HE | 0.931 | 0.931 | 0.853 | 0.929 | 0.931 | 0.947 | 0.941 | 0.905 | 0.879 | 0.941 | 0.939 | 0.909 | 0.903 | 0.929 | 0.931 |
HO | 0.828 | 0.660 | 0.556 | 0.577 | 0.860 | 0.706 | 0.726 | 0.580 | 0.667 | 0.648 | 0.686 | 0.689 | 0.680 | 0.591 | 0.583 |
FIS | 0.120 | 0.368 | 0.345 | 0.387 | 0.086 | 0.299 | 0.278 | 0.368 | 0.255 | 0.319 | 0.278 | 0.100 | 0.257 | 0.374 | 0.382 |
Pmin-11 | |||||||||||||||
n | 58 | 53 | 53 | 53 | 54 | 52 | 50 | 51 | 36 | 53 | 50 | 52 | 50 | 45 | 52 |
A | 7 | 5 | 6 | 7 | 6 | 7 | 7 | 6 | 5 | 6 | 5 | 5 | 6 | 7 | 6 |
HE | 0.596 | 0.543 | 0.518 | 0.552 | 0.598 | 0.587 | 0.598 | 0.585 | 0.157 | 0.513 | 0.439 | 0.623 | 0.550 | 0.533 | 0.470 |
HO | 0.552 | 0.566 | 0.547 | 0.547 | 0.648 | 0.592 | 0.700 | 0.412 | 0.167 | 0.418 | 0.460 | 0.712 | 0.600 | 0.511 | 0.500 |
FIS | 0.083 | 0.305 | −0.047 | −0.051 | −0.074 | −0.090 | −0.160 | 0.305 | −0.045 | 0.089 | −0.039 | −0.132 | −0.081 | 0.052 | −0.057 |
Total | |||||||||||||||
MNA | 28.57 | 24.00 | 20.00 | 23.57 | 24.57 | 23.00 | 24.29 | 24.00 | 15.14 | 26.57 | 24.29 | 20.00 | 22.14 | 22.57 | 22.43 |
HE | 0.859 | 0.840 | 0.813 | 0.849 | 0.844 | 0.846 | 0.834 | 0.862 | 0.743 | 0.843 | 0.814 | 0.827 | 0.828 | 0.813 | 0.817 |
HO | 0.720 | 0.676 | 0.636 | 0.705 | 0.761 | 0.764 | 0.726 | 0.676 | 0.659 | 0.702 | 0.726 | 0.615 | 0.713 | 0.743 | 0.693 |