Table 5 Simultaneous segregation at Aco-1 and the distyly locus

(a) BRY × Mhomo-H and reciprocal (not used in recombination frequency estimates)
	Aco-1 genotype
Morph	FF	FM	FS	MS
Short	0	0	8	2
Homostyle	0	9	0	0
Long	7	0	0	0
Total	7	9	8	2
Goodness of fit to 1:1:1:1 ratio, G_{3 df}=5.50, P=0.14
(b) Pooled backcrosses (S16L, S4L, K12L, K21L × Mhomo-H)
	Aco-1 genotype
	FM	FF
Homostyle	49	0
Long	0	100
Total	49	100
Goodness of fit to 1:1 ratio, G_{1 df}=17.8, P<0.001
G_{heterogeneity, 3 df}=0.74, P=0.86
r=0.007±0.007
(c) Krp12LMhom-4 H selfed and Mhomo-H selfed pooled
	Aco-1 genotype
	FF	FM	MM
Homostyle	3	38	22
Long	9	3	0
Total	12	41	22
Goodness of fit to 1:2:1 ratio, G₂=3.64, P=0.16
G_{heterogeneity, 2 df}=5.15, P=0.08
r=0.096+0.035
(d) S16L × Mhbry-9S
	Aco-1 genotype
	FM	FS
Short	5	128
Homostyle	35	1
Total	40	129
Goodness of fit to 1:1 ratio, G_{1 df}=49.3, P<0.001
r=0.0355+0.014
Joint recombination frequency, r=0.031±0.009, χ² _{heterogeneity, 3 df}=11.9, P=0.008

F, M and S are three alleles segregating at the Aco-1 locus. Goodness of fit tests to expected ratios at the Aco-1 locus are provided. Where crosses were pooled, a heterogeneity test is provided. Maximum likelihood estimates of the recombination frequency, r±SE are provided. Where no recombinants were observed, r was estimated upon the assumption that the next individual to be assayed would have been a recombinant. A joint estimate of the recombination frequency was obtained following Allard (1956).

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