Table 2 PCR primers and amplification conditions for codominant loci used in this study
From: First-generation linkage map of the warningly colored butterfly Heliconius erato
Locus | Primer name | Primers sequence | Annealing temp (°C) | Intron? |
|---|---|---|---|---|
Mpi | Mpi 14 | 5′-TTTAAGGTGCTCTATATAACRAARGC-3′ | 50 | Yes |
Mpi 15 | 5′-TTCTGGTTTGTGATTTGGATCYTTRTA-3′ | |||
Tpi | Tpi 1 | 5′-GGTCACTCTGAAAGGAGAACCATCTT-3′ | 50 | Yes |
Tpi 2 | 5′-CACAACATTTGCCCAGTTGTTGCCAA-3′ | |||
Wg | Wg F | 5′-CCCAGTTTTAGATCTGTCG-3′ | 50 | No |
Wg R | 5′-TCTCGGTCCGGTATCCGCG-3′ | |||
Dpp | Dpp F34 | 5′-AGAGAACGTGGCGAGACACTG-3′ | 55 | No |
Dpp R327 | 5′-GAGGAAAGTTGCGTAGGAACG-3′ | |||
Ci | Ci F190 | 5′-ATGCGGAGACATACTGGTGAA-3′ | 53 | Yes |
Ci R350 | 5′-GATGCTTAGCTCTGTCACTGG-3′ | |||
Ddc | Ddc F125 | 5′-CAAGCTCATTCGTCTGTCGAG-3′ | 57 | Yes |
Ddc R291 | 5′-CGAAAGCGCAAGACGATGTAG-3′ | |||
He-CA-001 | A25F | 5′-CGGGACACAAAATGTGACTG-3′ | 55 | N/A |
A25R | 5′-A-ACTGGACGGTGGACGTTAG-3′ | |||
Hel-01 | B6R | 5′-TCGTAGATATCCATTACTCTGGTCTG-3′ | 54 | N/A |
B6F | 5′-AGGGCGTCGTTAGTTTGTGT-3′ | |||
Hel-08 | A2F | 5′-ACATCTCAGAACTGGTCGGC-3′ | 55 | N/A |
A2R | 5′-CTCGATCAGCCGGTGATTAT-3′ | |||
Hel-13 | CA13F | 5′-ATTTCATAGTAACGCCCTCC-3′ | 52 | N/A |
CA13R | 5′-TGACTTATCGCTAAGGTCAA-3′ | |||
Hel-14 | AAT4F | 5′-GCACATTTACTTACACTAACGC-3′ | 53 | N/A |
AAT4R | 5′-ATTTGTTTCGAACGACTGCC-3′ | |||
Hel-04 | GA-8F | 5′-GGAACGGAGTGCCCTAAAAC-3′ | 55 | N/A |
GA-8R | 5′-CGTTGCCGCTTATACTTTCC-3′ | |||
Hel-05 | GA-11F | 5′-TGCTGTCCATACCCAACTCA-3′ | 55 | N/A |
GA-11R | 5′-CGAACTCACAACCATCAGTCA-3′ | |||
Hel-07 | GA-19R | 5′-CCCGAACCACTAAAGTCGAA-3′ | 55 | N/A |
GA-19F | 5′-GCGGGGACAACTACATAAGC-3′ | |||
Hel-11 | CA-10F | 5′-TTTCTTTTGAGTCCCGATGG-3′ | 55 | N/A |
CA-10R | 5′-ATCTCAGAACTGGTCGGCAG-3′ | |||
Hel-10 | CA-9F | 5′-TCTCACTTTCCCACACAGCA-3′ | 55 | N/A |
CA-9R | 5′-TGTGAAGAGACACATGGGGA-3′ | |||
Hel-03 | GA-7F | 5′-CCAATTATGTCACATGGATCTGTT-3′ | 54 | N/A |
GA-7R | 5′-CTCTGTCGTCCTCTGCAGTC-3′ | |||
Hel-06 | GA-16F | 5′-TAGCCTTCACTTTGAACCCG-3′ | 55 | N/A |
GA-16R | 5′-CCCACTCGAAGCAATGAAAT-3′ |
