Figure 4
From: Recent degeneration of an old duplicated flowering time gene in Brassica nigra
Detection of mRNA of B.nigra COb alleles by RT-PCR. RNA was isolated from leaves of B. nigra (Bn) and transformed A. thaliana plants (At) and used to synthesize cDNA, which was amplified by PCR using Cob-specific primers. Amplifications of 18S RNA were used as a control of cDNA amount and quality. To check for contamination of genomic DNA, amplifications were conducted on cDNA synthesis reactions both with (+ RT) and without reverse transcriptase (−RT). F and N denote apparently functional and nonfunctional alleles (see text).
