Table 6 Single-locus segregation ratios for test crosses and their reciprocals

Locus	Female heterozygous G		Male heterozygous G
1. MhBry-9S × S16L and reciprocal ^a
Aco-1	108 FM:131 FS	2.22^NS	40 FM:129 FS	49.3^***
S-locus	118 H:121 S	0.04^NS	36 H:133 S	52.9^***
Pgd-c	112 SS:127 FS	0.94^NS	35 SS:134 FS	61.8^***
TsPG	118 BK:121 BB	0.04^NS	40 BK:129 BB	49.3^***
Oph06a	89 Aa:150 aa	15.74^***	46 Aa:123 aa	37.6^***
Opi04a	89 Aa:150 aa	15.74^***	46 Aa:123 aa	37.6^***
Opi04b	119 Aa:120 aa	0.01^NS	83 Aa:86 aa	0.05^NS
Opk14a	109 Aa:130 aa	1.85^NS	81 Aa:86 aa	0.29^NS
2. SS3314-9S × S16L and SS3314-1S × S16L pooled and reciprocal (2005)
Aco-1	144 FF:144 FS	0.00^NS	147FF:141 FS	0.13^NS
Pgd-c	139 FS:149 SS	0.35^NS	143 FS:145 FS	0.01^NS

Abbreviation: NS, not significant.
For Aco-1 and Pgd-c, F, M, S, refer to alleles encoding fast-, intermediate- and slow-migrating allozymes. For the S-locus, S and H refer to short- and homostyled morphs. For the RAPD loci, Aa genotypes yield an amplified PCR band, whereas aa genotypes do not have the band.
The G-statistic for goodness of fit to a 1:1 ratio with 1 df is provided.
The pooled data for the reciprocal crosses of SS3314-9S × S16L and SS3314-1S × S16L were not statistically heterogeneous for either of the loci.
^aSegregation data for S16L × MhBry-9S for the first four loci were published in Athanasiou et al. (2003) and data for the S-locus for MhBry-9S × S16L were published in Tamari et al. (2005).
^***P< 0.001.

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