ABSTRACT
Xenopus organizer specific gene Noggin possesses nearly all the characterestic properties of the action of organizer to specify the embryonic body axis. To analyze how the maternal inherited factors control its expression pattern, we cloned the 5′ regulatory region of noggin gene. The 1.5 kb upstream sequense could direct reporter gene to express in vivo and data from deletion analysis indicated that a 229 base pair fragmet is essential for activating noggin expression. We further demonstrated that the response elements within this regulatory region were indeed under the control of growth factor activin and Wnt signaling pathway components.
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Acknowledgements
We are grateful to R.M. Harland for the psp64-activin bb and J Roose for pCDNA1.1-ArmdNXTcf-1 constructs. We also thank Mr. Huang Weigong for animal rearing. Ding XY was surported by National Foundation of Natural Science (39770356) and Fundation from Bureau of Basic Research, Chinese Academy of Sciences.
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TAO, Q., YANG, J., MEI, W. et al. Cloning and analysing of 5′ flanking region of Xenopus organizer gene noggin. Cell Res 9, 209–216 (1999). https://doi.org/10.1038/sj.cr.7290019
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DOI: https://doi.org/10.1038/sj.cr.7290019
