ABSTRACT
We reported in this manuscript that TGF-β1 induces apoptosis in AML12 murine hepatocytes, which is associated with the activation of p38 MAPK signaling pathway. SB202190, a specific inhibitor of p38 MAPK, strongly inhibited the TGF-β1-induced apoptosis and PAI-1 promoter activity. Treatment of cells with TGF-β1 activates p38. Furthermore, over-expression of dominant negative mutant p38 also reduced the TGF-β1-induced apoptosis. The data indicate that the activation of p38 is involved in TGF-β1-mediated gene expression and apoptosis.
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Abbreviations
- MAPK:
-
mitogen-activated protein kinase
- TGF-b:
-
transforming growth factorb
- TAK1:
-
TGF-b activated kinase 1
- JNK:
-
c-jun N-terminal kinase
- ERK:
-
extracellular signal-regulated kinase
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Acknowledgements
We are grateful to Dr. Rik Derynck for the TGF-β1, Dr. Joan MASSGÉ for providing PAI-1 reporter plasmid p3TP-Lux, Dr. Jia Huai HAN for the kind gifts of Plasmids of pcDNA3-Flag-p38, pcDNA3-Flag-p38(AF), He Hua CHEN for helpful discussion. This work was supported by grants from the Chinese Academy of Sciences (No. KJ951-B1-608), the National Natural Sciences Foundation of China (No. 39625007 and 39870396).
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LIAO, J., CHEN, J., CHAI, M. et al. The involvement of p38 MAPK in transforming growth factor β1-induced apoptosis in murine hepatocytes. Cell Res 11, 89–94 (2001). https://doi.org/10.1038/sj.cr.7290072
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DOI: https://doi.org/10.1038/sj.cr.7290072
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