ABSTRACT
The legume forage Alhagi pseudoalhagi was transformed by the Agrobacterium rhizogenes strain A4 using cotyledon and hypocotyl segments as infection materials. Plant regeneration was achieved from sterile calluses derived from hairy roots, which occurred at or near the infection sites. The hairy root regenerants were characterized by normal leaf morphology and stem growth but a shallow and more extensive root system than normal plants. Opine synthesis, PCR and Southern blot confirmed that T-DNA had been intergrated into the A. pseudoalhagi genome. Acetosyringone (AS) was found to be vital for successful transformation of A. pseudoalhagi.
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Abbreviations
- 6-BA:
-
Benzyladenine
- 2,4-d:
-
2,4-dichlorophe-noxyacetic
- IAA:
-
3-Indolylacetic acid
- IBA:
-
Indole-3-butyric acid
- NAA:
-
α-Naphthaleneacetic acid
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Acknowledgements
We express our sincere thanks to Lanzhou Institute of Desert Research, Chinese Academy of Sciences, for generously supplying mature seeds of A. pseudoalhagi. This research was supported by National Natural Science Foundation (No.30070366).
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WANG, Y., WANG, J., LUO, D. et al. Regeneration of plants from callus cultures of roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi. Cell Res 11, 279–284 (2001). https://doi.org/10.1038/sj.cr.7290097
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DOI: https://doi.org/10.1038/sj.cr.7290097
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