ABSTRACT
A systemic study was initiated to identify stage-specific expression genes in fish embryogenesis by using suppression subtractive hybridization (SSH) technique. In this study, we presented a preliminary result on screen for stage-specific expression genes between tail bud stage (TBS) and heartbeat beginning stage (HBS) in gynogenetic silver crucian carp ( Carassius auratus gibelio). Two SSH plasmid libraries specific for TBS embryos and HBS embryos were constructed, and stage-specific expression genes were screened between the two stages. 1963 TBS positive clones and 2466 HBS positive clones were sampled to PCR amplification, and 1373 TBS and 1809 HBS PCR positive clones were selected to carry out dot blots. 169 TBS dot blot positive clones and 272 HBS dot blot positive clones were sequenced. Searching GenBank by using these nucleotide sequences indicated that most of the TBS dot blot positive clones could not be found homologous sequences in the database, while known genes were mainly detected from HBS dot blot positive clones. Of the 79 known genes, 20 were enzymes or kinases involved in important metabolism of embryonic development. Moreover, specific expressions of partial genes were further confirmed by virtual northern blots. This study is the first step for making a large attempt to study temporal and spatial control of gene expression in the gynogenetic fish embryogenesis.
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References
Newport J W, Kirschner M W . Regulation of the cell cycle during Xenopus laevis development. Cell 1984; 37:731–42.
Wolpert L, Beddington R, Brockes J, Jessell T, Lawrence P, Meyerowitz E . Principles of Development. Current Biology Ltd 1998; London.
Sartgent TD, Dawid I . Differential gene expression in the gastrula of Xenopus laevis. Science 1983; 222:135–9.
Soriano P, Cone RD, Mulligan RC, Jaenisch R . Tissue-specific and ectopic of expression genes introduced into transgenic mice by retroviruses. Science 1986; 234:1409–13.
Driever W, Solnica-Krezel L, Schier AF, Neuhauss SC, Malicki J, Stemple DL, Stainer DY, Zwartkruis F, Abdelilah S, Rangini Z, Belak J, Boggs C . A genetic screen of mutations affecting mbryogenesis in zebrafish. Development 1996; 123:37–46.
Haffter P, Granato M, Brand M, Mullins MC, Hammerschmidt M, Kane DR, Odenthal J, van Eeden FJ, Jiang YJ, Heisenberg CP, Klsh RN, Furutani-Seiki M, Vogelsang E, Beuchle D, Schach U, Fabian C, Nusslein-Volhard C . The identification of genes with unique and essential functions in the development of zebrafish, Danio rerio. Development 1996; 123:1–36.
Diatchenko L, Lau YC, Campbell AP, Chenchik A, Mooadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov E, Siebert PD . Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes. Proc Natl Acad Sci USA 1996; 93:6025–30.
Chu ZL, Mckinsey TA . Suppression of tumor necrosis factor-induced cell death by inhibitor of apoptosis c-IAP2 is under NF-kB control. Proc Natl Acad Sci USA 1997; 94:10057–62.
Davis C A, Benzer S . Generation of cDNA expression libraries enriched for in-frame sequences. Proc Natl Acad Sci USA 1997; 94:2128–32.
Fan LC, Yang ST, Gui JF . Differential screening and characterization analysis of the egg envelop glycoprotein ZP3 cDNAs between genogenetic and gonochoristic crucian carp. Cell Research 2001; 11(1):7–27.
Xie J, Wen JJ, Chen B, Gui JF . Differential gene expression in full-grown oocytes between genogenetic and gonochoristic crucian carps. Gene 2001; 271:109–16.
Wen JJ, Xie J, Liu SG, Gui JF . Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis. Science in China 2001; 44:541–53.
Zhu ZY, Sun YH . Embryonic and genetic manipulation in fish. Cell Research 2000; 10(1):17–27.
Jiang YG, Liang SC, Chen BD, et al. Biological effect of heterologous sperm on gynogenetic offspring in Carassius auratus gibelio. Acta Hydrobiologica Sinica 1983; 8(1):1–13(in Chinese).
Yang ZA, Li QH, Wang YF, Gui JF . Comparative investigation on spindle behavior and MPF activity changes during oocyte maturation between genogenetic and amphimictic crucian carp. Cell Research 1999; 9:145–54.
Gui JF . A unique study system: gynogenetic fish Carassius auratus gibelio. Science Foundation in China 1996; 4(1):44–6.
Gui JF . Retrospects and prospects of studies on the mechanism of natural gynogenesis in silver crucian carp (Carassius auratus gibelio). Science Foundation in China 1997; 11:11–6(in Chinese).
Gui JF, Liang SC, Zhu LF, Jiang YG . Discovery of two different reproductive developmental modes of the eggs of artificial multiple tetraploid allogynogenetic silver crucian carp. Chinese science Bulletin 1993; 38:332–7.
Zhou L, Wang Y, Gui JF . Genetic evidence for gonochoristic reproduction in gynogenetic silver crucian carp (Carassius auratus gibelio Bloch) as revealed by RAPD assays. J Mol Evol 2000; 51:498–506.
Gui JF . Fish developmental genetics and artificial propagation. In: Wu C, Gui JF (eds). Fish genetics and breeding engineering. Shanghai Scientific and Technical Press, Shanghai, p41–66.
Duguin GL, Dinauer MC . Library subtraction of in vitro cDNA libraries to identify differential expressed genes in scrapie infection. Nucleic Acids Res 1990; 18:2789–92.
Lisitsyn N, Lisitsyn L, Wigler M . Cloning the differences between two complex genomes. Science 1993; 259:946–51.
Martin-Laurent F, Tuinen D . Differential display analysis of RNA accumulation in arbuscular mycorrhiza of pea and isolation of a noval symbiosis-regulated plant gene. Mol Gen Genet 1997; 256:37–44.
Xie J, Zhu Y, Zhang F, Gui JF . Differential gene expression of protein kinases in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp. Chinese Science Bulletin 1999; 44(14):1297–301.
Sompayac L, Jane S, Burn TC, Tenen DG, Danna KJ . Overcoming limitations of the mRNA differential display technique. Nucleic Acids Res 1995; 23:4738–9.
Hara E, Kato T, Nakada S, Sekiya S Oda K . Subtrctive cDNA cloning using oligo(dT)30-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells. Nucleic Acids Res 1991; 19:7097–104.
Hedrick SM, Cohen DI, Neilson EA, Davis MM . Isolation of cDNA clones encoding T cell-specific membrane-associated proteins. Nature 1984; 308:149–53.
Sargent TD, Dawid IB . Differential gene expression in the xenopus laevis. Science 1983; 222:135–9.
Diatchenko L, Lau YFC, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K Gurskaya N, Sverdlov ED, Siebert PD . Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci USA 1996; 93:6025–30.
Gurskaya NG, Diatchenko L, Chenchik A, Siebert PD, Khaspekov GL, Lukyanov K A, Vagner LL, Ermolaeva OD, Lukyanov SA, Sverdlov ED . Equalizing cDNA subtaction based on selective suppression of polymerase chain reaction: Cloning of Jurkat cell transcripts induced by phytohemaglutinin and phorbol 12-myristate 13-acetate. Anal Biochem 1996; 240:90–7.
Endege WO, Steinmann KE, Boardman LA, Thibodeau SN, Schlegel R . Representative cDNA libraries and their utility in gene expression profiling. Bio Techniques 1999; 26:542–50.
Thisse C, Zon LI . Organogenesis-heart and blood formation from the zebrafish point of view. Science 2002; 295:457–62.
Brownlie A, Zon L . The zebrafish as a model system for the study of hematopoiesis. BioScience 1999; 49:382–92.
Orkin SH, Zon LI . Hematopoiesis and sterm cells: plasticity versus developmental heterogeneity. Nature Immunology 2002; 3:323–8.
Sadlon TJ, Dell'Oso T, Surinya KH, May BK . Regulation of erythroid 5-aminolevulinate synthase expression during erythropoiesis. Int J Biochem Cell Biol 1999; 31(10):1153–67.
Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant No. 30130240), the Chinese Academy of Sciences (Grant No. KSCX2-SW-303).
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SHI, Y., LIU, J., XIA, J. et al. Screen for stage-specific expression genes between tail bud stage and heartbeat beginning stage in embryogenesis of gynogenetic silver crucian carp. Cell Res 12, 133–142 (2002). https://doi.org/10.1038/sj.cr.7290119
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DOI: https://doi.org/10.1038/sj.cr.7290119
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