ABSTRACT
p28, a 28kD protein from toad (Bufo bufo gargarizans) oocytes, was identified by using p13suc1-agarose affinity chromatography. Sequence homology analysis of the full-length cDNA of p28(Gene Bank accession number: AF 314091) indicated that it encodes a protein containing 224 amino-acids with about 55% identities and more than 70% positives to human, rat or mouse UCH-L1, and contains homological functional domains of UCH family. Anti-p28 monoclonal antibody, on injecting into the oocytes, could inhibit the progesterone-induced resumption of meiotic division in a dose-dependent manner. The recombinant protein p28 showed similar SDS/PAGE behaviors to the native one, and promoted ubiquitin ethyl ester hydrolysis, a classical catalytic reaction for ubiquitin carboxyl terminal hydrolases (UCHs). The results in this paper reveal that a novel protein, p28, exists in the toad oocytes, is a UCH L1 homolog, was engaged in the process of progesterone-induced oocyte maturation possibly through an involvement in protein turnover and degradation.
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This work is supported by National Natural Science Fundation of China (Grant 39770370), and National Laboratory of Contraceptives and Devices Research affiliated with Shanghai Institute of Planned Parenthood Research.
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SUN, Z., KONG, W., ZHANG, Y. et al. A novel ubiquitin carboxyl terminal hydrolase is involved in toad oocyte maturation. Cell Res 12, 199–206 (2002). https://doi.org/10.1038/sj.cr.7290125
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DOI: https://doi.org/10.1038/sj.cr.7290125
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