Abstract
The role of inositol 1,4,5-trisphosphate (IP3) in transducing heat-shock (HS) signals was examined in Arabidopsis. The whole-plant IP3 level increased within 1 min of HS at 37 °C. After 3 min of HS, the IP3 level reached a maximum 2.5 fold increase. Using the transgenic Arabidopsis plants that have AtHsp18.2 promoter-β-glucuronidase (GUS) fusion gene, it was found that the level of GUS activity was up-regulated by the addition of caged IP3 at both non-HS and HS temperatures and was down-regulated by the phospholipase C (PLC) inhibitors {1-[6-((17β-3-Methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl]-2,5-pyrrolidinedione}(U-73122).
The intracellular-free calcium ion concentration ([Ca2+]i) increased during HS at 37 °C in suspension-cultured Arabidopsis cells expressing apoaequorin. Treatment with U-73122 prevented the increase of [Ca2+]i to some extent. Above results provided primary evidence for the possible involvement of IP3 in HS signal transduction in higher plants.
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Acknowledgements
We are grateful to Professor MR Knight (Department of Plant Sciences, University of Oxford, Oxford, UK) for the suspension-culture Arabidopsis cells expressing apoaequorin. We thank Dr Takahashi and Professor Yoshibumi Komeda (Molecular Genetics Research Laboratory, The University of Tokyo, Tokyo, Japan) for the transgenic Arabidopsis seeds. This work was supported by the National Natural Science Foundation of China (No. 30270796) and Natural Science Foundation of Hebei Province, China (No. C2005000171).
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Liu, H., Gao, F., Cui, S. et al. Primary evidence for involvement of IP3 in heat-shock signal transduction in Arabidopsis. Cell Res 16, 394–400 (2006). https://doi.org/10.1038/sj.cr.7310051
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DOI: https://doi.org/10.1038/sj.cr.7310051
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