Figure 2
Sensitivity of the cells to FasL and TRAIL. (a) [35S] methionine labeled target cells (5 × 105 cells/ml) were coincubated with FasL-expressing cells (39.5-B7-FasL) or with nonexpressor (39.5-B7) for 18 h and release of radioactivity was monitored. L1210-fas cells were used as a positive control. The data represent average±SD of triplicate in one of three independent experiments. (b) Expression of mTRAIL receptor (DR5) on parental D122 and cytokine transfectants (NAT.A, DIF-2 and DIL6-7) was tested according to Materials and methods. (c) Cells (1 × 106 cells) were incubated with sTRAIL (100 ng/ml) overnight, and cells were analyzed by flow cytometry after staining with Annexin and PI. The data shows one of two independent experiments.
