Abstract
Twenty-one expressed sequence tags (ESTs) belonging to unidentified transcripts were mapped to 9 intervals at 11p15.5-p15.3. Thirteen were mapped to a region of an estimated size of 7 Mb with the help of a YAC contig. The remaining eight were mapped outside this region, and the centromeric or telomeric position of the ESTs relative to the YAC contig was defined with the help of a cellular hybrid containing a derivative chromosome 11 with a breakpoint within the region covered by the contig. The average size of the intervals, where the ESTs were mapped, was estimated to be 0.3–0.4 Mb. The refinement of the chromosomal location of these ESTs could be of great help in the identification of potential candidate genes for disease locus mapping at 11p15.
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Acknowledgements
We thank Marco Campi for technical assistance. This work was supported by grants from Telethon (grant E366), the Associazione Italiana per la Ricerca sul Cancro and from Consiglio Nazionale delle Ricerche, Progetto Finalizzato ‘Applicazioni Cliniche della Ricerca Oncologica’.
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Schwienbacher, C., Sabbioni, S., Barbanti-Brodano, G. et al. Refined Subchromosomal Location of 21 Expressed Sequence Tags from Unknown Genes at Region 11p15. Eur J Hum Genet 5, 214–217 (1997). https://doi.org/10.1007/BF03405920
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DOI: https://doi.org/10.1007/BF03405920
