Figure 1 | EMBO reports

Figure 1

From: Toll-like receptor 9 protects non-immune cells from stress by modulating mitochondrial ATP synthesis through the inhibition of SERCA2

Figure 1

Identification of SERCA2 as a binding protein of toll-like receptor 9 (TLR9).

A Representative image of tandem affinity purification visualized by silver stain is presented. Tandem affinity purification was performed using cardiomyocytes that were adenovirally transfected with TLR9-HA-FLAG or YFP-HA-FLAG. Cardiac fibroblasts transfected with TLR9-HA-FLAG were used as a control. MW: molecular weight markers. Note that the 95-kDa band marked by an asterisk was observed in TLR9 immunoprecipitates from cardiomyocytes, but not from cardiac fibroblasts.

B CpG-ODN increased the target protein binding to TLR9 (asterisk) in cardiomyocytes.

Source data are available online for this figure.

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