Figure 6
From: Conformation and physics of polymer chains: a single-molecule perspective
Mapping of the emission sites within a single polymer chain. (a) Principle of super-resolution localization microscopy. The emission intensity profile from one molecule is fitted using a 2D Gaussian function (left) to determine the centroid position (right). (b) Fluorescence intensity trajectory of a single MEH–PPV molecule embedded in a thin film of Zeonex. Each intensity level is represented by a different color. (c) Left: Centroid positions obtained from the trajectory in (b). Large circles show positions of emitting sites. The radii of circles correspond to standard deviations of the centroid positions. Each color represents the position obtained for the intensity level of the same color as in (b). Right: Schematic conformation of the molecule. Emitting sites within the molecule are indicated by light-blue dots.
