Figure 5
The measurement of viability and intracellular reactive oxidative species (ROS) of A549 cells. (a) The cell viability after treatment with carbon nanotube (CNT)-1 and CNT-3. Note: The x axis represents the concentration of CNTs. (b) The correlation between cell viability and the content of metal residues in CNTs. (c) The cell viability after treatment with concentrated leachates (leachate-AIF (artificial intestinal fluid), leachate-ALF (artificial lysosomal fluid), leachate-AGF (artificial gastric fluid)). (d) The cell viability after treatment with 10 μg ml−1 of CNT-2, c-leachate (from CNT-2 incubation in AGF for 2 months) and a mixture of both. (e) Representative confocal microscope images showing the generation of intracellular ROS after respective treatment with concentrated leachates (c-leachate-ALF, c-leachate-AIF, c-leachate-AGF). (f) Representative confocal microscope images showing the generation of intracellular ROS and actin cytoskeleton organization after respective treatment for 24 h with 10 μg ml−1 of CNT-2, c-leachate-AGF and a mixture of both.
