Figure 4
From: Prebiotic-chemistry inspired polymer coatings for biomedical and material science applications
Surface analysis, quantification and biological evaluation of silver-treated AMN coatings. (a) Atomic Ag/C ratios derived from quantification of XPS survey spectra. (b) Auger spectra of three different surfaces with increasing silver surface density showing the increasing presence of metallic silver as the surface density increases. (c) Correlation of atomic Ag/C ratio determined by XPS with the absolute amount, if silver incorporated, determined by ICP-OES. (d) 24 h biofilm assay-based semi-quantitative crystal violet staining. The two representative bacterial strains, S. epidermidis (Gram-positive) and P. aeruginosa (Gram negative), show a concentration-dependent decrease in biofilm formation with biofilm mass for both strains sharply dropping of above concentrations of 10−5 M AgNO3 used for incubating the AMN coatings. AMN, aminomalononitrile; ICP-OES, inductively coupled plasma optical emission spectroscopy; XPS, X-ray photoelectron spectroscopy.
