Figure 3
Site-specific and programmable drug-DNA adduct formation. (a) Average drug copy numbers per polynucleotide in drug-DNA adduct prepared from doxorubicin and polynucleotides (A20, G20, C20 and T20), indicating that deoxyguanine had the highest reactivity for drug-DNA adduct formation. This provides a guideline to programmably design DNA and drugs for drug-DNA adduct-based drug delivery systems. (b) Drug-DNA adduct formation with doxorubicin and various DNA sequences. (c) Drug-DNA adduct formation using DNA (sgc8-5 T) with commonly used daunorubicin and enhanced permeability and retention, respectively. Purified drug-DNA adducts were quantified based on absorbance at 506 nm, which was normalized to that of the corresponding control. (Control: reaction using sgc8-5T without formaldehyde).
