Figure 6
Representative images of tissue analyses verifying drug-aptamer adducts-induced potent antitumor efficacy and reduction of side effects. (a and b) Hematoxylin and eosin histopathological analysis of tumors (a) and hearts (b) indicating tumor necrosis in mice treated with free doxorubicin or drug-aptamer adducts, as well as reduction of heart tissue deformation in mice treated with drug-aptamer adducts, compared with free doxorubicin. (c and d) In situ immunohistochemical analysis of cleaved Caspase-3 (brown) in tumors (c) and hearts (d) indicating tumor apoptosis in mice treated with free doxorubicin or drug-aptamer adducts, but no detectable apoptosis in hearts from mice treated with drug-aptamer adducts, compared with free doxorubicin (arrows indicate apoptosis). Blue: cell nuclei stained by hematoxylin. (scale bar: 200 μm).
